New Chromogenic Spray Reeagent for Detection of Acephate from Biological Material
U. K. Kulkarni1, K. V. Kulkarni2, D. V. Mane3, R. K. Pardeshi4
1U. K. Kulkarni, Directorate of Forensic Laboratory, State of Maharashtra, Mumbai, India.
2K. V. Kulkarni, Directorate of Forensic Laboratory, State of Maharashtra, Mumbai, India.
3D. V. Mane, Dr. Babasaheb Ambedkar Marathwada University Aurangabad, India. R. K. Pardeshi, S. R. College Ghansangavi, Jalna, India.
Manuscript received on February 01, 2015. | Revised Manuscript Received on February 09, 2015. | Manuscript published on February 20, 2015. | PP: 1-2 | Volume-3 Issue-4, March 2015. | Retrieval Number: D0593033415/2015©BEIESP
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Abstract: High performance thin layer chromatography has found wide recognition in many fields and in sensitivity of detection offers particular advantage to the toxicologist, which has increased, 10 to 100 times as compared to the chemical method. It has become an important analytical tool since it can separate complex mixture in a relatively short time. In existing study, an effort has been taken to determine organ phosphorus insecticide Acephate by using high performance thin layer chromatography. A new specific Sensitive chromogenic reagent 0.1% solution of ferric chloride in 80% ethanol and 1 % Sulfosalicylic acid in 80% ethanol has been developed for detection of Acephate an organophosphorus insecticide with solvent system petroleum ether: methanol (95: 5).
Keywords: Chromatography, chromogenic, Acephate, ferric chloride, ethanol, Sulfosalicylic, organophosphorus.